The 5’ ends of the selected fragments will form groups on the positive- and negative-strand. Peak calling, the next step in our workflow, is a computational method used to identify areas in the genome that have been enriched with aligned reads as a consequence of performing a ChIP-sequencing experiment.įor ChIP-seq experiments, what we observe from the alignment files is a strand asymmetry with read densities on the +/- strand, centered around the binding site. List and describe the output files from MACS2.Describe the parameters involved in running MACS2.Describe the different components of the MACS2 peak calling algorithm. Peak calling with MACS2 | In-depth-NGS-Data-Analysis-Course In-depth-NGS-Data-Analysis-Course View on GitHubĬontributors: Meeta Mistry, Radhika KhetaniĪpproximate time: 80 minutes Learning Objectives
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