The 5’ ends of the selected fragments will form groups on the positive- and negative-strand. ![]() Peak calling, the next step in our workflow, is a computational method used to identify areas in the genome that have been enriched with aligned reads as a consequence of performing a ChIP-sequencing experiment.įor ChIP-seq experiments, what we observe from the alignment files is a strand asymmetry with read densities on the +/- strand, centered around the binding site. List and describe the output files from MACS2.Describe the parameters involved in running MACS2.Describe the different components of the MACS2 peak calling algorithm. ![]() Peak calling with MACS2 | In-depth-NGS-Data-Analysis-Course In-depth-NGS-Data-Analysis-Course View on GitHubĬontributors: Meeta Mistry, Radhika KhetaniĪpproximate time: 80 minutes Learning Objectives
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